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Establishment of an miR‑137‑knockout cell model using ...

The pXPR001-sgRNA plasmids were verified using sequencing and integrated into the genome of the ovarian cancer cell line, A2780, through lentiviral trans-duction, puromycin selection and single-cell culture. PCR products amplified from single‑cell cultures using primers
www.spandidos-publications.com/10.3892/ol.2018.909...

Lenti‐Cas9‐2A‐Blast | Addgene 73310 product informationhttps://www.labome.com/product/Addgene/73310.htmlbackbone: lenti CRISPR pXPR_001: backbone_mutation: Cas9-2A Blasticidin vector was modified from lentiCRISPR pXPR_001 (Addgene 49535) by removing the gRNA expressing sequence through NdeI, EcoRI digest and blunt end ligation using large Klenow fragments (NEB) to ligate sticky ends. 2A- puro was replaced with an amplified 2A-blasticidin sequence with NheI and MluI sites

backbone: lenti CRISPR pXPR_001: backbone_mutation: Cas9-2A Blasticidin vector was modified from lentiCRISPR pXPR_001 (Addgene 49535) by removing the gRNA expressing sequence through NdeI, EcoRI digest and blunt end ligation using large Klenow fragments (NEB) to ligate sticky ends. 2A- puro was replaced with an amplified 2A-blasticidin sequence with NheI and MluI sites
www.labome.com/product/Addgene/73310.html

Details and Download Full Text PDF: Establishment of an ...https://www.pubfacts.com/detail/30128024/Establishment-of-an-miR...Oncol Lett 2018 Sep 6;16(3):4027-4032. Epub 2018 Jul 6. Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong ...

Oncol Lett 2018 Sep 6;16(3):4027-4032. Epub 2018 Jul 6. Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong ...
www.pubfacts.com/detail/30128024/Establishment-of-...

Establishment of an miR‑137‑knockout cell model using ...https://www.spandidos-publications.com/10.3892/ol.2018.9096The pXPR001‑sgRNA plasmids were verified using sequencing and integrated into the genome of the ovarian cancer cell line, A2780, through lentiviral transduction, puromycin selection and …

The pXPR001‑sgRNA plasmids were verified using sequencing and integrated into the genome of the ovarian cancer cell line, A2780, through lentiviral transduction, puromycin selection and …
www.spandidos-publications.com/10.3892/ol.2018.909...

Addgene: pXPR_011https://www.addgene.org/59702Plasmid pXPR_011 from Dr. David Root's lab contains the insert EGFP sgRNA and is published in Nat Biotechnol. 2014 Sep 3. doi: 10.1038/nbt.3026. This plasmid is available through Addgene.

Plasmid pXPR_011 from Dr. David Root's lab contains the insert EGFP sgRNA and is published in Nat Biotechnol. 2014 Sep 3. doi: 10.1038/nbt.3026. This plasmid is available through Addgene.
www.addgene.org/59702

Exome Sequencing of African-American Prostate Cancer ...cancerdiscovery.aacrjournals.org/content/7/9/973Abstract. African-American men have the highest incidence of and mortality from prostate cancer. Whether a biological basis exists for this disparity remains unclear.

Abstract. African-American men have the highest incidence of and mortality from prostate cancer. Whether a biological basis exists for this disparity remains unclear.
cancerdiscovery.aacrjournals.org/content/7/9/973

Establishment of an miR‑137‑knockout cell model using ...https://www.researchgate.net/publication/326233756_Establishment...MicroRNA-137 (miR-137) has been reported to be abnormally expressed in a variety of types of cancer, including ovarian cancer. However, the roles served by miR-137 in cancer are not fully ...

MicroRNA-137 (miR-137) has been reported to be abnormally expressed in a variety of types of cancer, including ovarian cancer. However, the roles served by miR-137 in cancer are not fully ...
www.researchgate.net/publication/326233756_Establi...

Addgene: lentiCRISPR - EGFP sgRNA 1https://www.addgene.org/51760lentiCRISPR - EGFP sgRNA 1 was a gift from Feng Zhang (Addgene plasmid # 51760) For your References section: Genome-scale CRISPR-Cas9 knockout screening in human cells .

lentiCRISPR - EGFP sgRNA 1 was a gift from Feng Zhang (Addgene plasmid # 51760) For your References section: Genome-scale CRISPR-Cas9 knockout screening in human cells .
www.addgene.org/51760

LentiCRISPRv2 and lentiGuide-Puro: lentiviral CRISPR/Cas9 ...

Page 1 of 2 rev20140722 LentiCRISPRv2 and lentiGuide-Puro: lentiviral CRISPR/Cas9 and single guide RNA CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is a microbial nuclease system involved in defense against invading phages and plasmids.
genome-engineering.org/gecko/wp-content/uploads/20...

Yuanli He's research works | Southern Medical University ...https://www.researchgate.net/scientific-contributions/58751642...The pXPR001-sgRNA plasmids were verified using sequencing and integrated into the genome of the ovarian cancer cell line, A2780, through lentiviral transduction, puromycin selection and single ...

The pXPR001-sgRNA plasmids were verified using sequencing and integrated into the genome of the ovarian cancer cell line, A2780, through lentiviral transduction, puromycin selection and single ...
www.researchgate.net/scientific-contributions/5875...